High-efficiency electrospray is critical to achieving the full potential of mass spectrometry. The CO2 can undergo phase conversion to supercritical fluid at P > 7.3 MPa and T > 31.1 oC (sCO2). The supercritical fluids provide fast analysis, high throughput, and improved resolution. Nanospraying the supercritical fluid provides efficient desolvation and therefore higher ionization efficiency. We developed a split-flow system that providing equal ionization opportunities for analytes in a complex matrix. This benefits for accurate quantification with higher metabolomic and lipidomic coverage than conventional LC.
Supercritical fluid sCO2 nanospray mass spectrometry.
3D Heat map signal intensity of tagged hexanal, octanal, and decanal as a function of linear velocity and pressure.