Our focus is on how the metabolism of endothelial cells which are relevant to vascular diabetic complications is malfunctioning. Characterization of metabolic changes may serve as a key component for pathway activation and biomarkers for vascular complications of diabetes mellitus.

The MS signals depend on which functional groups the metabolite contains. In order to overcome this difficulty in quantitation, we proposed chemical tagging (derivatization). This allows for improving the proton affinity moiety and diminish competing ionization. Given the shortcomings of current therapies for diabetic complications and the inherent metabolic implications of the disease, untargeted metabolomics will serve to uncover novel pathways and biomarkers.